Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
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The chimeric genes in pSB 130 expression vectors were transformed into Agrobacterium tumefaciens EHA105 by electroporation. |
After two weeks inducation, calli of japonica cv. 9983 were used for rice transformation. Agrobacterium-mediated transformation, selection and regeneration were performed following the protocol provided by CAMBIA (http://www.cambia.org/daisy/cambia/4214.html). T2 seeds generated from positive T1 plants were used for protein expression analysis and purification experiments. |
| Protocol tips |
| The chimeric genes in pSB 130 expression vectors were transformed into Agrobacterium tumefaciens EHA105 by electroporation. |
| Downstream tips |
| After two weeks inducation, calli of japonica cv. 9983 were used for rice transformation. Agrobacterium-mediated transformation, selection and regeneration were performed following the protocol provided by CAMBIA (http://www.cambia.org/daisy/cambia/4214.html). T2 seeds generated from positive T1 plants were used for protein expression analysis and purification experiments. |
Publication protocol
The chimeric genes in pSB 130 expression vectors were transformed into Agrobacterium tumefaciens EHA105 by electroporation. After two weeks inducation, calli of japonica cv. 9983 were used for rice transformation. Agrobacterium-mediated transformation, selection and regeneration were performed following the protocol provided by CAMBIA (http://www.cambia.org/daisy/cambia/4214.html). Regenerated transgenic rice plantlets were transferred to soil and grown in facilities for transgenic plants at The Chinese University of Hong Kong. Positive transgenic rice plants were determined by PCR screening and Southern blot analysis. Mature positive transgenic rice seeds were collected as T1 seeds. T2 seeds generated from positive T1 plants were used for protein expression analysis and purification experiments.
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