Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
| This vector includes a strong, thermo-inducible Pr promoter [31] and was co-operating with CI repressor. To improve the success rate of plasmid construction, a specific CI repressor-mutant B. subtilis strain BUSY 9797 was employed, which totally represses the expression of inserted genes. |
The copy number of pGETS118 vector in B. subtilis tends to be close to one but can be increased by 1 mM isopropyl 1-thio-β-d-galactoside (IPTG). The resulting plasmids were re-purified and transformed into the expression host B. subtilis RM125. |
|
| Upstream tips |
| This vector includes a strong, thermo-inducible Pr promoter [31] and was co-operating with CI repressor. To improve the success rate of plasmid construction, a specific CI repressor-mutant B. subtilis strain BUSY 9797 was employed, which totally represses the expression of inserted genes. |
| Protocol tips |
| The copy number of pGETS118 vector in B. subtilis tends to be close to one but can be increased by 1 mM isopropyl 1-thio-β-d-galactoside (IPTG). The resulting plasmids were re-purified and transformed into the expression host B. subtilis RM125. |
Publication protocol
The copy number of pGETS118 vector in B. subtilis tends to be close to one but can be increased by 1 mM isopropyl 1-thio-β-d-galactoside (IPTG). This vector includes a strong, thermo-inducible Pr promoter [31] and was co-operating with CI repressor. To improve the success rate of plasmid construction, a specific CI repressor-mutant B. subtilis strain BUSY 9797 was employed, which totally represses the expression of inserted genes. The resulting plasmids were re-purified and transformed into the expression host B. subtilis RM125.
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