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The recombinant pEGFP-INHα plasmid was purified and recovered. The plasmid was mixed with Lipofectamine 2000 at a ratio of 4:1 and incubated with BHK cells at 37 °C in the presence of 5% CO2. After 48 h, over 50% of the cells were positive for green fluorescent protein (GFP), as determined under an inverted epifluorescence microscope. |
Cells were harvested and divided into two groups, which were subjected to RT-PCR and Western blot analysis, respectively. |
| Protocol tips |
| The recombinant pEGFP-INHα plasmid was purified and recovered. The plasmid was mixed with Lipofectamine 2000 at a ratio of 4:1 and incubated with BHK cells at 37 °C in the presence of 5% CO2. After 48 h, over 50% of the cells were positive for green fluorescent protein (GFP), as determined under an inverted epifluorescence microscope. |
| Downstream tips |
| Cells were harvested and divided into two groups, which were subjected to RT-PCR and Western blot analysis, respectively. |
Publication protocol
The recombinant pEGFP-INHα plasmid was purified and recovered. The plasmid was mixed with Lipofectamine 2000 at a ratio of 4:1 and incubated with BHK cells at 37 °C in the presence of 5% CO2. After 48 h, over 50% of the cells were positive for green fluorescent protein (GFP), as determined under an inverted epifluorescence microscope. Cells were harvested and divided into two groups, which were subjected to RT-PCR and Western blot analysis, respectively.
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