pXMJ19-xynA

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	The pXMJ19-xynA and pEC-XK99E-xynA plasmids were then transformed into the C. glutamicum mutants alone or together for XynA expression.

Protocol tips
The pXMJ19-xynA and pEC-XK99E-xynA plasmids were then transformed into the C. glutamicum mutants alone or together for XynA expression.

Publication protocol

To analyze the effect of host mutation on XynA production, mutations of the cell surface layer protein CspB2 and the protease ClpS were constructed. The protease ClpS (encoded by clpS, GenBank: CP025533.1)-disrupted mutant ΔclpS was constructed based on homologous recombination in the lab strain. The other mutations were constructed by homologous recombination as described previously [13]. The pK18mobSacB-cspB2 vector was transformed into C. glutamicum CGMCCl.15647, the resulting mutant was designated ΔcspB2 after single-crossover and double-crossover selection. Then, the cspB2 and clpS double mutation was obtained using the same method and designated as ΔcspB2ΔclpS. Then, pK18mobSacB-xynA for integration of xynA into the chromosome was transformed into ΔcspB2 and ΔcspB2ΔclpS, resulting in the desired xynA-integrated mutants ΔcspB2InX and ΔcspB2ΔclpSInX, respectively. The pXMJ19-xynA and pEC-XK99E-xynA plasmids were then transformed into the C. glutamicum mutants alone or together for XynA expression.

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