WST-1 Cell Proliferation Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Absorbance was measured at a wavelength of 450 nm.

Downstream tips
- Absorbance was measured at a wavelength of 450 nm.

Publication protocol

Cell attachment and proliferation was measured using a WST-1 assay kit (Clontech). The WST-1 assay used to determine cell viability is based on the reductive cleavage of tetrazolium salt to the soluble formazan by a mitochondrial dehydrogenase that is active only in viable cells. The amount of dye formed was immediately measured by a microplate reader (Biotek uQuant) at a wavelength of 450 nm. At each time point, four samples were used to measure the number of cells attached to the electrospun matrices. The experiments were performed in triplicate.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - MG-63 using WST-1 Cell Proliferation Assay Kit from Takara Bio Inc.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for WST-1 Cell Proliferation Assay Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - MG-63 using WST-1 Cell Proliferation Assay Kit from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.