CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cella were seeded at a density of 1×104 cells per well.	- The cells were then treated with cisplatin alone or in combination with emodin at the indicated concentration for 24, 36 and 48 h, respectively.

Upstream tips
- Cella were seeded at a density of 1×104 cells per well.
Protocol tips
- The cells were then treated with cisplatin alone or in combination with emodin at the indicated concentration for 24, 36 and 48 h, respectively.

Publication protocol

The cell proliferation assay was conducted using a colorimetric method with an MTS assay kit (CellTiter 96®Aqueous Non-Radioactive Cell Proliferation Assay; Promega Corporation, Madison, WI, USA). In brief, the MG-63 cells in the logarithmic phase of growth were seeded into a 96-well plate at a density of 1×104 cells per well, 24 h prior to treatment. The cells were then treated with cisplatin (0.1, 0.2, 0.4, 0.8, 1.6, 3.2 µM) alone or in combination with emodin (5, 10, 20, 40, 80 and 100 µM) at the indicated concentration for 24, 36 and 48 h, respectively. The production of formazan was read at 490 nm using Flexstation 3 (Molecular Devices, Sunnyvale, CA, USA). The intensity of the color produced was proportional to the number of living cells

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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) below.

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