Protocol tips
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Protocol tips |
Downstream tips |
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Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14]. |
Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ). |
| Protocol tips |
| Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14]. |
| Downstream tips |
| Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ). |
Publication protocol
Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14]. Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ).
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pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 from Adam F. Barrios, Center for Eukaryotic Gene Regulation, Departme has not yet been reviewed for this experiment
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Papers
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