pST50Trc3-yGcn5

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14].	Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ).

Protocol tips
Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14].
Downstream tips
Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ).

Publication protocol

"Expression and metal affinity purification: Small scale (100 ml) expression in E. coli and small scale purification of the tagged proteins or protein complexes were performed as described [14]. Western blotting was performed using anti-Gcn5 antibodies (custom antibodies by Cocalico Biologicals, Reamstown, PA) or anti-HIS antibodies (GenScript, Piscataway, NJ).
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Manufacturer protocol

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