Cell Counting Kit-8

Protocol tips

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	- Cell number was quantified at days 0 (day of seeding), 1, 2 and 3

Protocol tips
- Cell number was quantified at days 0 (day of seeding), 1, 2 and 3

Publication protocol

Cells were seeded into 96-well plates (Corning, NY, USA) in triplicate at a density of 1×103 cells per well with RPMI-1640 containing 10% FBS. The cell number was quantified at days 0 (day of seeding), 1, 2 and 3 with the Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assay, and the cell growth curves were plotted with the cell number values as ordinate and time as abscissa. Each experiment was performed in triplicate.

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Manufacturer protocol

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