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E. coli (DH5α) cells transformed with pGB2B3 and pGEX-4T-1 were induced with isopropyl-β-D-thiogalactopyra-noside (IPTG) to express the fusion BD2/3 protein which was purified on a GST affinity column (Amersham Biosciences; GE Healthcare). |
The bioactivity of the fusion protein was measured by inhibition of 4 standard pathogen strains (E. coli ATCC25922, S. aureus ATCC 26112, S. pneumoniae ATCC49619 and P. aeruginosa ATCC10211). |
| Protocol tips |
| E. coli (DH5α) cells transformed with pGB2B3 and pGEX-4T-1 were induced with isopropyl-β-D-thiogalactopyra-noside (IPTG) to express the fusion BD2/3 protein which was purified on a GST affinity column (Amersham Biosciences; GE Healthcare). |
| Downstream tips |
| The bioactivity of the fusion protein was measured by inhibition of 4 standard pathogen strains (E. coli ATCC25922, S. aureus ATCC 26112, S. pneumoniae ATCC49619 and P. aeruginosa ATCC10211). |
Publication protocol
E. coli (DH5α) cells transformed with pGB2B3 and pGEX-4T-1 were induced with isopropyl-β-D-thiogalactopyra-noside (IPTG) to express the fusion BD2/3 protein which was purified on a GST affinity column (Amersham Biosciences; GE Healthcare). The bioactivity of the fusion protein was measured by inhibition of 4 standard pathogen strains (E. coli ATCC25922, S. aureus ATCC 26112, S. pneumoniae ATCC49619 and P. aeruginosa ATCC10211).
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