pTY- α-amylase

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells.

Protocol tips
The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells.

Publication protocol

The reaction was performed using 10 ng DNA, 2 μL (10 μM) of each primer, 5 μL (10mM) dNTPs, 5 μL 10× PCR buffer [100mM Tris-HCl (pH 8.9), 500mM KCl, 20mM MgCl2, 1% Triton X-100] and High-fidelity PCR Enzyme Mix (Fermentas). After 2 minutes of preliminary heating at 95°C in a thermal cycler, each of the 30 cycles was conducted at 95°C for 0.5 minutes, 56°C for 1 minute, and 72°C for 1 minute, with a final step of 5 minutes at 72°C. The amplified gene was cloned into pTZ57R/T (InsTAclone PCR Cloning Kit; Fermentas), and then subcloned into pTYB2 and transformed into E. coli BL21 (DE3) pLysS cells.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase using pTY- α-amylase from Nourkhoda Sadeghifard, Clinical Microbiology Research Center, Il.

Manufacturer protocol

Download the product protocol from Nourkhoda Sadeghifard, Clinical Microbiology Research Center, Il for pTY- α-amylase below.

Videos

Check out videos that might be relevant for performing Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase using pTY- α-amylase from Nourkhoda Sadeghifard, Clinical Microbiology Research Center, Il. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.