Cytotoxicity Detection KitPLUS (LDH)

Cell cytotoxicity / Proliferation assay cell type - HT-22

Experiment
Cell cytotoxicity / Proliferation assay cell type - HT-22
Product
Cytotoxicity Detection KitPLUS (LDH) from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Cell supernantant was assayed after 24h of treatement with reagents.

Publication protocol

To evaluate damaged HT-22 cells in these experiments, the release of LDH was quantitated in the culture medium using the Cytotoxicity Detection Kitplus (Roche Applied Bioscience, Indianapolis, IN, USA) following the manufacturer’s protocol. Following 24 h of the indicated cell treatment, medium was collected for analysis of LDH. After subtracting the background values in the medium, the percentage cytotoxicity was calculated with the following equation: LDH release (% of Max) = 100 × (experimental value − low control)/(high control − low control). Experimental value, LDH values in the experimental groups; Low control, LDH values in the untreated normal cells; High control, the maximum releasable LDH values in the untreated normal cells.

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Papers

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Paper title
Homer1a attenuates glutamate-induced oxidative injury in HT-22 cells through regulation of store-operated calcium entry
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cytotoxicity Detection KitPLUS (LDH) below.

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