OmpA-TRAIL/pET-22b

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The recombinant plasmid OmpA-TRAIL/pET22b was transformed into the E.coli BL21 (DE3) by treatment with 0.1 M cold CaCl2 and then heat shock at 42 °C for 1 minute. The transformed bacteria was cultured in Terrific Broth (TB) medium (1.2% peptone; 2.4% yeast extract; 72 mM K2HPO4; 17 mM KH2PO4; and 0.4% glycerol; pH 7.2) on a rotary shaker (200 rpm) at 37 °C until the optical density at 600 nm (OD600) of the culture reached 0.6. Then, isopropyl-β-D-thiogalacto-pyran-oside (IPTG) was added to a final concentration of 0.1 mM to induce the expression of the target protein until 24 h.

Protocol tips

The recombinant plasmid OmpA-TRAIL/pET22b was transformed into the E.coli BL21 (DE3) by treatment with 0.1 M cold CaCl2 and then heat shock at 42 °C for 1 minute. The transformed bacteria was cultured in Terrific Broth (TB) medium (1.2% peptone; 2.4% yeast extract; 72 mM K2HPO4; 17 mM KH2PO4; and 0.4% glycerol; pH 7.2) on a rotary shaker (200 rpm) at 37 °C until the optical density at 600 nm (OD600) of the culture reached 0.6. Then, isopropyl-β-D-thiogalacto-pyran-oside (IPTG) was added to a final concentration of 0.1 mM to induce the expression of the target protein until 24 h.

Publication protocol

The recombinant plasmid OmpA-TRAIL/pET22b was transformed into the E.coli BL21 (DE3) by treatment with 0.1 M cold CaCl2 and then heat shock at 42 °C for 1 minute. The transformed bacteria was cultured in Terrific Broth (TB) medium (1.2% peptone; 2.4% yeast extract; 72 mM K2HPO4; 17 mM KH2PO4; and 0.4% glycerol; pH 7.2) on a rotary shaker (200 rpm) at 37 °C until the optical density at 600 nm (OD600) of the culture reached 0.6. Then, isopropyl-β-D-thiogalacto-pyran-oside (IPTG) was added to a final concentration of 0.1 mM to induce the expression of the target protein until 24 h.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - E. coli TRAIL using OmpA-TRAIL/pET-22b from Bahram Kazem, Cellular and Molecular Biology Research Center, Sh.

Manufacturer protocol

Download the product protocol from Bahram Kazem, Cellular and Molecular Biology Research Center, Sh for OmpA-TRAIL/pET-22b below.

Videos

Check out videos that might be relevant for performing Protein Expression Prokaryotic cells - E. coli TRAIL using OmpA-TRAIL/pET-22b from Bahram Kazem, Cellular and Molecular Biology Research Center, Sh. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.