pET-PH0380

Protocol tips

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	E. coli BL21-CodonPlus(DE3)-RIL harboring pET-PH0380 was cultured in LB medium until reaching an OD600 of 0.5. After IPTG (isopropyl-β-d-thiogalactopyranoside) was added to a final concentration of 1 mM, cultures were further incubated for 4 h.

Protocol tips
E. coli BL21-CodonPlus(DE3)-RIL harboring pET-PH0380 was cultured in LB medium until reaching an OD600 of 0.5. After IPTG (isopropyl-β-d-thiogalactopyranoside) was added to a final concentration of 1 mM, cultures were further incubated for 4 h.

Publication protocol

E. coli BL21-CodonPlus(DE3)-RIL harboring pET-PH0380 was cultured in LB medium until reaching an OD600 of 0.5. After IPTG (isopropyl-β-d-thiogalactopyranoside) was added to a final concentration of 1 mM, cultures were further incubated for 4 h.

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