Cell Proliferation ELISA, BrdU

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- 3×104 cells/cm2 cells were seeded in six-well plate. - Assay was performed in serum-free MEM supplemented with or without different test substances with different incubation periods.

Upstream tips
- 3×104 cells/cm2 cells were seeded in six-well plate. - Assay was performed in serum-free MEM supplemented with or without different test substances with different incubation periods.

Publication protocol

3×104 cells/cm2 cells were seeded in six-well plate, cultured for 24 h and serum starved for 16 h prior to the experiment. Experiments were performed in serum-free MEM supplemented with or without 10 ng/ml Fgf2 for 24 h. The same experiment was repeated in the presence of 30 mM NaClO3 with or without Fgf2, after 6 h pretreatment of the cells with 30 mM NaClO3. Cells were pretreated with the FgfR inhibitor PD173074 (20 nM) (Sigma Aldrich) for 6 h, followed by 24 h incubation with 20 nM PD173074 with or without Fgf2 (Welti et al., 2011). Working concentrations for Fgf2, NaClO3 and PD173074 were determined based on titration curves. Proliferation experiments were evaluated by cell counting or BrdU incorporation (Cell Proliferation ELISA, Roche Applied Science).

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