pET28a(+)-rBPL2

Protein Expression Prokaryotic cells - E. coli rBPL2

Experiment

Protein Expression Prokaryotic cells - E. coli rBPL2

Product

pET28a(+)-rBPL2 from Jong Won Ha, Department of Genetic Resources Research, National

Manufacturer

Jong Won Ha, Department of Genetic Resources Research, National

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	E. coli BL21(λDE3) cells (Invitrogen) were transformed by pET28a: BPL2. The transformants were spread on Luria-Bertani (LB) agar plates containing 25 μg/mL kanamycin. Positive colonies were isolated and subcultured in 10 mL LB-kanamycin medium.

Protocol tips
E. coli BL21(λDE3) cells (Invitrogen) were transformed by pET28a: BPL2. The transformants were spread on Luria-Bertani (LB) agar plates containing 25 μg/mL kanamycin. Positive colonies were isolated and subcultured in 10 mL LB-kanamycin medium.

Publication protocol

E. coli BL21(λDE3) cells (Invitrogen) were transformed by pET28a: BPL2. The transformants were spread on Luria-Bertani (LB) agar plates containing 25 μg/mL kanamycin. Positive colonies were isolated and subcultured in 10 mL LB-kanamycin medium.

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Papers

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Manufacturer protocol

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