pS2E8Y

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Upstream tips	Protocol tips	Downstream tips
	As shown in Figure 4a, the expression plasmid of SELPs, named S2E8Y and S4E8Y, were constructed using our previously established procedures and expressed under the T7 promoter in E. coli strain BL21Star (DE3) (Invitrogen, Carlsbad, CA, USA) in a New Brunswick BioFlo 3000 bioreactor (New Brunswick Scientific, Edison, NJ, USA) [59].	The protein was purified by the inverse transition cycling (ITC) method, as described previously [7]. The purity of the proteins was monitored via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular weight of SELPs were determined by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF).

Protocol tips
As shown in Figure 4a, the expression plasmid of SELPs, named S2E8Y and S4E8Y, were constructed using our previously established procedures and expressed under the T7 promoter in E. coli strain BL21Star (DE3) (Invitrogen, Carlsbad, CA, USA) in a New Brunswick BioFlo 3000 bioreactor (New Brunswick Scientific, Edison, NJ, USA) [59].
Downstream tips
The protein was purified by the inverse transition cycling (ITC) method, as described previously [7]. The purity of the proteins was monitored via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular weight of SELPs were determined by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF).

Publication protocol

As shown in Figure 4a, the expression plasmid of SELPs, named S2E8Y and S4E8Y, were constructed using our previously established procedures and expressed under the T7 promoter in E. coli strain BL21Star (DE3) (Invitrogen, Carlsbad, CA, USA) in a New Brunswick BioFlo 3000 bioreactor (New Brunswick Scientific, Edison, NJ, USA) [59]. The protein was purified by the inverse transition cycling (ITC) method, as described previously [7]. The purity of the proteins was monitored via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular weight of SELPs were determined by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF).

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