pET31b(+)- LPcin-YK3

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	The recombinant protein containing a ketosteroid isomerase (KSI) fusion partner and His6-tag to facilitate purification, was expressed in a mutant Escherichia coli C41(DE3) strain (Novagen, Darmstadt, Germany), as described previously [11, 14]. The cells were cultured in Luria-Bertani (LB) medium containing carbenicillin and transferred to M9 minimal medium and induced with 1 mmol/l isopropyl β-D-1-thiogalactopyranoside (IPTG, Amresco, USA) addition.	The LPcin-YK3 peptide was finally purified by reverse-phase high-pressure liquid chromatography (RP-HPLC) (Waters Binary 1525, USA).

Protocol tips
The recombinant protein containing a ketosteroid isomerase (KSI) fusion partner and His6-tag to facilitate purification, was expressed in a mutant Escherichia coli C41(DE3) strain (Novagen, Darmstadt, Germany), as described previously [11, 14]. The cells were cultured in Luria-Bertani (LB) medium containing carbenicillin and transferred to M9 minimal medium and induced with 1 mmol/l isopropyl β-D-1-thiogalactopyranoside (IPTG, Amresco, USA) addition.
Downstream tips
The LPcin-YK3 peptide was finally purified by reverse-phase high-pressure liquid chromatography (RP-HPLC) (Waters Binary 1525, USA).

Publication protocol

The recombinant protein containing a ketosteroid isomerase (KSI) fusion partner and His6-tag to facilitate purification, was expressed in a mutant Escherichia coli C41(DE3) strain (Novagen, Darmstadt, Germany), as described previously [11, 14]. The cells were cultured in Luria-Bertani (LB) medium containing carbenicillin and transferred to M9 minimal medium and induced with 1 mmol/l isopropyl β-D-1-thiogalactopyranoside (IPTG, Amresco, USA) addition. The LPcin-YK3 peptide was finally purified by reverse-phase high-pressure liquid chromatography (RP-HPLC) (Waters Binary 1525, USA).

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