pACLYC

Protocol tips

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	Four colonies were picked from agar plates, inoculated into 2xPY medium (20 g/L Peptone, 10 g/L Yeast extract, and 10 g/L NaCl, pH = 7) containing 34 μg/mL Chloramphenicol and 100 μg/mL Ampicillin, and incubated overnight. Ten micro-liter aliquots of overnight grown cell culture were inoculated into 1 mL 2xPY medium in 14 mL BD Falcon™ tube. Cells were grown at 37°C with shaking (300rpm) till OD595 reached the range of 0.5~1.0. The cells were then induced with various concentrations of L-Arabinose/IPTG and grown at 28°C or 37°C for indicated time before collected for RNA/protein extraction or Lycopene assay.

Protocol tips

Four colonies were picked from agar plates, inoculated into 2xPY medium (20 g/L Peptone, 10 g/L Yeast extract, and 10 g/L NaCl, pH = 7) containing 34 μg/mL Chloramphenicol and 100 μg/mL Ampicillin, and incubated overnight. Ten micro-liter aliquots of overnight grown cell culture were inoculated into 1 mL 2xPY medium in 14 mL BD Falcon™ tube. Cells were grown at 37°C with shaking (300rpm) till OD595 reached the range of 0.5~1.0. The cells were then induced with various concentrations of L-Arabinose/IPTG and grown at 28°C or 37°C for indicated time before collected for RNA/protein extraction or Lycopene assay.

Publication protocol

Four colonies were picked from agar plates, inoculated into 2xPY medium (20 g/L Peptone, 10 g/L Yeast extract, and 10 g/L NaCl, pH = 7) containing 34 μg/mL Chloramphenicol and 100 μg/mL Ampicillin, and incubated overnight. Ten micro-liter aliquots of overnight grown cell culture were inoculated into 1 mL 2xPY medium in 14 mL BD Falcon™ tube. Cells were grown at 37°C with shaking (300rpm) till OD595 reached the range of 0.5~1.0. The cells were then induced with various concentrations of L-Arabinose/IPTG and grown at 28°C or 37°C for indicated time before collected for RNA/protein extraction or Lycopene assay.

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