pT7lacO2

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	For lycopene expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium containing the appropriate inducer and grown at 37°C and 180 rpm for 18 hours. For time-course experiments, overnight cultures were diluted to an OD of 0.15 in 50 mL of fresh medium, the appropriate inducer was added, and the sample was aliquoted into culture tubes corresponding to different time points. OD and lycopene content were measured at the time of inoculation, every hour for six hours, and at 18 hours.

Protocol tips

For lycopene expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium containing the appropriate inducer and grown at 37°C and 180 rpm for 18 hours. For time-course experiments, overnight cultures were diluted to an OD of 0.15 in 50 mL of fresh medium, the appropriate inducer was added, and the sample was aliquoted into culture tubes corresponding to different time points. OD and lycopene content were measured at the time of inoculation, every hour for six hours, and at 18 hours.

Publication protocol

For lycopene expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium containing the appropriate inducer and grown at 37°C and 180 rpm for 18 hours. For time-course experiments, overnight cultures were diluted to an OD of 0.15 in 50 mL of fresh medium, the appropriate inducer was added, and the sample was aliquoted into culture tubes corresponding to different time points. OD and lycopene content were measured at the time of inoculation, every hour for six hours, and at 18 hours.

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