pDG101

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	To test independent induction of two genes, GFPmut2 in pJS102 was replaced by mScarlet-I (amplified from a plasmid [19]) to make pDG101. Plasmids were cotransformed into MG1655 by electroporation according to the above protocol, except with 1 μl (each) undiluted plasmid (20 to 40 ng) and selection on LB agar plates with both spectinomycin and carbenicillin. Sequence maps are included in an online repository (33), and plasmids useful for constructing additional two-gene expression systems (pJS101 and pJS102) (Table 1) are available from Addgene (deposit no. 118280 and 118281) and have been verified by whole-plasmid sequencing (34).

Protocol tips

To test independent induction of two genes, GFPmut2 in pJS102 was replaced by mScarlet-I (amplified from a plasmid [19]) to make pDG101. Plasmids were cotransformed into MG1655 by electroporation according to the above protocol, except with 1 μl (each) undiluted plasmid (20 to 40 ng) and selection on LB agar plates with both spectinomycin and carbenicillin. Sequence maps are included in an online repository (33), and plasmids useful for constructing additional two-gene expression systems (pJS101 and pJS102) (Table 1) are available from Addgene (deposit no. 118280 and 118281) and have been verified by whole-plasmid sequencing (34).

Publication protocol

To test independent induction of two genes, GFPmut2 in pJS102 was replaced by mScarlet-I (amplified from a plasmid [19]) to make pDG101. Plasmids were cotransformed into MG1655 by electroporation according to the above protocol, except with 1 μl (each) undiluted plasmid (20 to 40 ng) and selection on LB agar plates with both spectinomycin and carbenicillin. Sequence maps are included in an online repository (33), and plasmids useful for constructing additional two-gene expression systems (pJS101 and pJS102) (Table 1) are available from Addgene (deposit no. 118280 and 118281) and have been verified by whole-plasmid sequencing (34).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - E. coli mScarlet-I using pDG101 from Zach Hensel, Instituto de Tecnologia Química e Biológica Antó.

Manufacturer protocol

Download the product protocol from Zach Hensel, Instituto de Tecnologia Química e Biológica Antó for pDG101 below.

Videos

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