Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
|
Fresh transformants were obtained by streaking M15 bacterial culture containing subcloned guinea pig TNF-α in pQE-30 vector on Luria-Bertini (LB) agar plates containing 100 μg/mL ampicillin (Sigma, St. Louis, MO) and 100 μg/mL kanamycin (Sigma). |
|
| Protocol tips |
| Fresh transformants were obtained by streaking M15 bacterial culture containing subcloned guinea pig TNF-α in pQE-30 vector on Luria-Bertini (LB) agar plates containing 100 μg/mL ampicillin (Sigma, St. Louis, MO) and 100 μg/mL kanamycin (Sigma). |
Publication protocol
The cloning of guinea pig TNF-α was accomplished by using the Concanavalin A-stimulated guinea pig splenocytes as described previously [24]. The construct containing coding sequence of guinea pig TNF-α was a generous gift from Dr. Teizo Yoshimura, National Cancer Institute, USA. The mature peptide region of guinea pig TNF-α (accession number-AF119622) was subcloned into the BamHI and HindIII site of pQE-30 vector (Qiagen, Chatsworth, CA) and transformed with M15 competent cells as described previously by our group [17]. Fresh transformants were obtained by streaking M15 bacterial culture containing subcloned guinea pig TNF-α in pQE-30 vector on Luria-Bertini (LB) agar plates containing 100 μg/mL ampicillin (Sigma, St. Louis, MO) and 100 μg/mL kanamycin (Sigma).
Full paper
Login or
join for free to view the full paper.
Reviews
pQE-30 from David N. McMurray, Department of Microbial Pathogenesis and Immu has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha using pQE-30 from David N. McMurray, Department of Microbial Pathogenesis and Immu.
Manufacturer protocol
Download the product protocol from David N. McMurray, Department of Microbial Pathogenesis and Immu for pQE-30 below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha using pQE-30 from David N. McMurray, Department of Microbial Pathogenesis and Immu. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.