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The competent cells, Escherichia coli MC1061, were grown in Luria-Bertani (LB) medium for cloning of the plasmids at 37 °C with shaking. Chloramphenicol (Cm) was utilized at a final concentration 10 μg/mL when it was necessary. |
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| Protocol tips |
| The competent cells, Escherichia coli MC1061, were grown in Luria-Bertani (LB) medium for cloning of the plasmids at 37 °C with shaking. Chloramphenicol (Cm) was utilized at a final concentration 10 μg/mL when it was necessary. |
Publication protocol
Lactobacillus casei CC16 was isolated from the intestine of common carp, and grown in De Man, Rogosa and Sharpe (MRS) medium (Thermo Fisher Scientific, Oxoid, UK) at 30 °C without shaking. The Escherichia coli-Lactobacillus shuttle vector pPG-1 and pPG-2 have been described in our previous study [44]. The competent cells, Escherichia coli MC1061, were grown in Luria-Bertani (LB) medium for cloning of the plasmids at 37 °C with shaking. Chloramphenicol (Cm) was utilized at a final concentration 10 μg/mL when it was necessary. Aeromonas veronii TH0426 strain was isolated from the farmed yellow catfish Pelteobagrus fulvidraco as described by Kang et al. [45].
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