pET28a-FSHR

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	The construct plasmid pET28a-FSHR was then transformed in E. coli strain BL21 Star™ (DE3). After confirmation by DNA sequencing, the positive clones were cultured in Luria broth medium (0.5% w/v yeast extract, 1% (w/v) tryptone, 1% (w/v) NaCl) with 50 mg l−1 kanamycin at 37°C and shaken at 200 rpm for 4 h until the absorbance at 600 nm (A600 nm) reached 1.2.

Protocol tips
The construct plasmid pET28a-FSHR was then transformed in E. coli strain BL21 Star™ (DE3). After confirmation by DNA sequencing, the positive clones were cultured in Luria broth medium (0.5% w/v yeast extract, 1% (w/v) tryptone, 1% (w/v) NaCl) with 50 mg l−1 kanamycin at 37°C and shaken at 200 rpm for 4 h until the absorbance at 600 nm (A600 nm) reached 1.2.

Publication protocol

The construct plasmid pET28a-FSHR was then transformed in E. coli strain BL21 Star™ (DE3). After confirmation by DNA sequencing, the positive clones were cultured in Luria broth medium (0.5% w/v yeast extract, 1% (w/v) tryptone, 1% (w/v) NaCl) with 50 mg l−1 kanamycin at 37°C and shaken at 200 rpm for 4 h until the absorbance at 600 nm (A600 nm) reached 1.2.

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