pgMAX system-DsRed2

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	Cell culture and lipofection were performed as described previously [11]. Human embryonic kidney cells (HEK293, ATCC CRL 1573) were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. Exponentially growing cells were plated onto 35-mm dishes, and lipofection was performed using commercially prepared lipofectamine (Invitrogen).

Protocol tips
Cell culture and lipofection were performed as described previously [11]. Human embryonic kidney cells (HEK293, ATCC CRL 1573) were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. Exponentially growing cells were plated onto 35-mm dishes, and lipofection was performed using commercially prepared lipofectamine (Invitrogen).

Publication protocol

Cell culture and lipofection were performed as described previously [11]. Human embryonic kidney cells (HEK293, ATCC CRL 1573) were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. Exponentially growing cells were plated onto 35-mm dishes, and lipofection was performed using commercially prepared lipofectamine (Invitrogen).

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