pONE-40A

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	HEK293 mammalian cells were transfected with the mixture of branched polyethylene-imine (No. 408727, Sigma-Aldrich) and pONE-40A (or pONE-41A) constructs (ratio 3:1) at 5×105 cell/mL density in 2 mL Dulbecco’s Modified Eagle’s Medium containing 10% FBS (Fetal Bovine Serum) and penicillin-streptomycin antibiotic. Transfected cells were incubated for 72 hours at 37°C in the presence of 5% carbon-dioxide. After incubation, cells were separated from the medium for further analysis.

Protocol tips

HEK293 mammalian cells were transfected with the mixture of branched polyethylene-imine (No. 408727, Sigma-Aldrich) and pONE-40A (or pONE-41A) constructs (ratio 3:1) at 5×105 cell/mL density in 2 mL Dulbecco’s Modified Eagle’s Medium containing 10% FBS (Fetal Bovine Serum) and penicillin-streptomycin antibiotic. Transfected cells were incubated for 72 hours at 37°C in the presence of 5% carbon-dioxide. After incubation, cells were separated from the medium for further analysis.

Publication protocol

HEK293 mammalian cells were transfected with the mixture of branched polyethylene-imine (No. 408727, Sigma-Aldrich) and pONE-40A (or pONE-41A) constructs (ratio 3:1) at 5×105 cell/mL density in 2 mL Dulbecco’s Modified Eagle’s Medium containing 10% FBS (Fetal Bovine Serum) and penicillin-streptomycin antibiotic. Transfected cells were incubated for 72 hours at 37°C in the presence of 5% carbon-dioxide. After incubation, cells were separated from the medium for further analysis.

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