pVRB2B3

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	The complexes of nanoparticles containing CS, PEG-O-CS-PEI, LP, PCL and PCL-protamine each containing 5 µg VRB2B3 plasmid were added into each well to transfect cells, respectively. Then, the transfected cells were incubated for 48 h and the supernatants were collected for antibacterial bioactivity detection (E. coli and S. Pneumoniae) of fusion BD2/3.

Protocol tips
The complexes of nanoparticles containing CS, PEG-O-CS-PEI, LP, PCL and PCL-protamine each containing 5 µg VRB2B3 plasmid were added into each well to transfect cells, respectively. Then, the transfected cells were incubated for 48 h and the supernatants were collected for antibacterial bioactivity detection (E. coli and S. Pneumoniae) of fusion BD2/3.

Publication protocol

93 cells (human embryonic kidney cells; ATCC no. CRL-1573TM) were purchased from the Chinese Academy of Science Cell bank (Shanghai, China). 293 cells were cultured in 6 well plates (1.5×106 cells/well) for 24 h and grown in 2 ml Dulbecco's modified Eagle's medium (DMEM; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 4.0 mM L-glutamine, 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin (Thermo Fisher Scientific, Inc.), and maintained at 37°C in a 5% CO2 humidified incubator (Sanyo Electric Co., Ltd., Tokyo, Japan) until the cell confluency of 293 achieved 80%. The complexes of nanoparticles containing CS, PEG-O-CS-PEI, LP, PCL and PCL-protamine each containing 5 µg VRB2B3 plasmid were added into each well to transfect cells, respectively. Then, the transfected cells were incubated for 48 h and the supernatants were collected for antibacterial bioactivity detection (E. coli and S. Pneumoniae) of fusion BD2/3.

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