Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
|
For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C. |
|
| Protocol tips |
| For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C. |
Publication protocol
For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C.
Full paper
Login or
join for free to view the full paper.
Reviews
pUC19/Pr-EKL from Christopher H. Taron, New England Biolabs, Ipswich has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Eukaryotic cells - K. lactis Enterokinase using pUC19/Pr-EKL from Christopher H. Taron, New England Biolabs, Ipswich.
Manufacturer protocol
Download the product protocol from Christopher H. Taron, New England Biolabs, Ipswich for pUC19/Pr-EKL below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Protein Expression Eukaryotic cells - K. lactis Enterokinase using pUC19/Pr-EKL from Christopher H. Taron, New England Biolabs, Ipswich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.