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For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C. |
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| Protocol tips |
| For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C. |
Publication protocol
For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C.
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