pUC19/Pr-GLuc

Protein Expression Eukaryotic cells - K. lactis Gaussia luciferase

Experiment
Protein Expression Eukaryotic cells - K. lactis Gaussia luciferase
Product
pUC19/Pr-GLuc from Christopher H. Taron, New England Biolabs, Ipswich
Manufacturer
Christopher H. Taron, New England Biolabs, Ipswich

Protocol tips

Protocol tips
For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C.

Publication protocol

For expression in yeast, a purified linearized expression cassette (0.1 μg) was introduced into K. lactis GG799 competent cells, as described by the manufacturer (New England Biolabs). Transformants were selected by growth on nitrogen-free yeast carbon base (YCB) agar medium (New England Biolabs) supplemented with 5 mM acetamide for 3 to 4 days at 30°C.

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Papers

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Manufacturer protocol

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