DeadEnd™ Colorimetric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Store the Equilibration Buffer, rTdT Enzyme, Biotinylated Nucleotide Mix and Proteinase K at –20°C		- If high background, Increase blocking time with 0.3% hydrogen peroxide

Upstream tips
- Store the Equilibration Buffer, rTdT Enzyme, Biotinylated Nucleotide Mix and Proteinase K at –20°C
Downstream tips
- If high background, Increase blocking time with 0.3% hydrogen peroxide

Publication protocol

Formalin-fixed tumor samples were paraffin-embedded and used for IHC analyses of PCNA and phospho-H2A.X (Ser139) following the earlier described methods (25). The apoptotic cell population in the tumor sections was determined by DeadEnd Colorimetric TUNEL assay kit (Promega, Madison, WI). The percent positive stained cells were determined as number of positive cells (brown stained) × 100/total number of cells in five randomly selected area per tumor section at ×400 magnification, and images were processed by Zeiss AxioVision software documentation system (Carl Zeiss, Jena, Germany).

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

Download manufacturer protocol

Videos

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