pSS1-cMSP33D7

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Five-ten colonies from each transformation were grown overnight at 30 °C in 5 ml LAB medium supplemented with 4% glycerol–phosphate, 5% glucose and 5 μg/ml erythromycin.

Protocol tips
Five-ten colonies from each transformation were grown overnight at 30 °C in 5 ml LAB medium supplemented with 4% glycerol–phosphate, 5% glucose and 5 μg/ml erythromycin.

Publication protocol

"All constructs were verified by sequencing and subsequently transformed into Lactococcus lactis MG1363 by electroporation as described [22].
Five-ten colonies from each transformation were grown overnight at 30 °C in 5 ml LAB medium supplemented with 4% glycerol–phosphate, 5% glucose and 5 μg/ml erythromycin. Culture supernatants were clarified by centrifugation at 9000g for 20 min and protein expression levels were assessed in the culture supernatants by ELISA using HRP-conjugated anti-His antibody (MACS, Miltenyi biotech, Germany) and by SDS-PAGE. L. lactis MG1363 harboring expression constructs was grown in a 1 l stirred bioreactors for 15 h at 30 °C [23]. Cells were removed by centrifugation at 9000 rpm for 30 min and the raw culture supernatant was clarified by filtration. Culture-filtrates were either used immediately or stored at − 20 °C."

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - L. lactis cMSP33D7 P. falciparum using pSS1-cMSP33D7 from Michael Theisen, Centre for Medical Parasitology at Department o.

Manufacturer protocol

Download the product protocol from Michael Theisen, Centre for Medical Parasitology at Department o for pSS1-cMSP33D7 below.

Videos

Check out videos that might be relevant for performing Protein Expression Prokaryotic cells - L. lactis cMSP33D7 P. falciparum using pSS1-cMSP33D7 from Michael Theisen, Centre for Medical Parasitology at Department o. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.