ApopTag® Peroxidase In Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- 5 x 106 cells/mL can be seeded for an assay and fixed with 1% paraformaldehyde in PBS, pH 7.4, for 10 minutes - 3.0% hydrogen peroxide was used for quenching. -

Protocol tips
- 5 x 106 cells/mL can be seeded for an assay and fixed with 1% paraformaldehyde in PBS, pH 7.4, for 10 minutes - 3.0% hydrogen peroxide was used for quenching. -

Publication protocol

TdT-mediated dUTP nick end labeling (TUNEL) was performed with an ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions. The number of TUNEL-positive cells was counted and averaged across 10 random fields from 7 mice per group.

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Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - FaDu human squamous cell carcinoma using ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Millipore.

Manufacturer protocol

Download the product protocol from Millipore for ApopTag® Peroxidase In Situ Apoptosis Detection Kit below.

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