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The Leukocyte cell-free protein synthesis system is comprised of leukocyte cell lysate and solutions for the Reaction Mix and Dialysis Buffer4,5,11. All components were allowed to come to room temperature. The components were then added in the following order: 50 µL lysate, 20 µL 5 × reaction mix, 0.4 µg GADD34 plasmid, and 4 µg cDNA plasmid. The mixture is brought to a total volume of 100 µL with nuclease-free water. |
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| Protocol tips |
| The Leukocyte cell-free protein synthesis system is comprised of leukocyte cell lysate and solutions for the Reaction Mix and Dialysis Buffer4,5,11. All components were allowed to come to room temperature. The components were then added in the following order: 50 µL lysate, 20 µL 5 × reaction mix, 0.4 µg GADD34 plasmid, and 4 µg cDNA plasmid. The mixture is brought to a total volume of 100 µL with nuclease-free water. |
Publication protocol
The Leukocyte cell-free protein synthesis system is comprised of leukocyte cell lysate and solutions for the Reaction Mix and Dialysis Buffer4,5,11. All components were allowed to come to room temperature. The components were then added in the following order: 50 µL lysate, 20 µL 5 × reaction mix, 0.4 µg GADD34 plasmid, and 4 µg cDNA plasmid. The mixture is brought to a total volume of 100 µL with nuclease-free water.
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