pGSA1285/CBD‐DrsB1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Two A. rhizogenes (ATCC15834) clones harboring the pGSA1285/CBD‐DrsB1 and pGSA1285/DrsB1‐CBD expression vectors were used to inoculate tobacco leaf disks. Briefly, sterilized 3‐week‐old tobacco leaf disks (1 cm2) were cut and put in an A. rhizogenes two‐day inoculation suspension for 10 min. The inoculated leaves were dried using a sterile filter paper and then placed on the hormone and antibiotic‐free MS culture medium.

Protocol tips

Two A. rhizogenes (ATCC15834) clones harboring the pGSA1285/CBD‐DrsB1 and pGSA1285/DrsB1‐CBD expression vectors were used to inoculate tobacco leaf disks. Briefly, sterilized 3‐week‐old tobacco leaf disks (1 cm2) were cut and put in an A. rhizogenes two‐day inoculation suspension for 10 min. The inoculated leaves were dried using a sterile filter paper and then placed on the hormone and antibiotic‐free MS culture medium.

Publication protocol

Tobacco (Nicotiana tabacum) Xanthi cultivar seeds were disinfected in a detergent solution (5% sodium hypochlorite and Triton X‐100) for 10 min and then washed three times with distilled water to remove detergent residues. The seeds were then germinated on MS culture medium under 16 hr light/8 hr dark photoperiod at 24 ± 2°C. Two A. rhizogenes (ATCC15834) clones harboring the pGSA1285/CBD‐DrsB1 and pGSA1285/DrsB1‐CBD expression vectors were used to inoculate tobacco leaf disks. Briefly, sterilized 3‐week‐old tobacco leaf disks (1 cm2) were cut and put in an A. rhizogenes two‐day inoculation suspension for 10 min. The inoculated leaves were dried using a sterile filter paper and then placed on the hormone and antibiotic‐free MS culture medium. The inoculated leaf disks were incubated at 24 ± 2°C for 2–3 days in dark and were eventually transferred to the selective medium containing kanamycin (50 mg/L) and cefotaxime (200 mg/L) for HR induction. The explants were regularly subcultured once every 2 weeks until root formation (Tempe & Casse‐Delbart, 2012).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Eukaryotic cells - N. benthamiana DrsB1 using pGSA1285/CBD‐DrsB1 from Farhad Nazarian‐Firouzabadi, Agronomy and Plant Breeding Depar.

Manufacturer protocol

Download the product protocol from Farhad Nazarian‐Firouzabadi, Agronomy and Plant Breeding Depar for pGSA1285/CBD‐DrsB1 below.

Videos

Check out videos that might be relevant for performing Protein Expression Eukaryotic cells - N. benthamiana DrsB1 using pGSA1285/CBD‐DrsB1 from Farhad Nazarian‐Firouzabadi, Agronomy and Plant Breeding Depar. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.