in situ Cell Death Detection Kit, POD

TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Experiment
TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma
Product
in situ Cell Death Detection Kit, POD from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Add Tunel mixture and incubate for 60 min at +37°C in a humidified atmosphere in the
dark
Downstream tips
If no specific labelling, reduce concentration of TdT by diluting it 1:2 up to 1:10 with TUNEL Dilution Buffer.

If high background, reduce concentration of labeling mix
to 50% by diluting with TUNEL Dilution Buffer

Publication protocol

Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay kit (in situ Cell Death Detection Kit, Beyotime Institute of Biotechnology, China) was used to assess the number of apoptotic cells. Frozen sections were fixed in 4% paraformaldehyde in pH 7.4 PBS for 1 h at room temperature and permeabilized in PBS with 1.0% Triton X-100 for 2 minutes. The broken DNA ends of dead cells were labelled using TUNEL kit following the manufacturer’s protocol.


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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using in situ Cell Death Detection Kit, POD from Sigma-Aldrich.

Paper title
ENTPD5 Induces Apoptosis in Lung Cancer Cells via Regulating Caspase 3 Expression
Full paper
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for in situ Cell Death Detection Kit, POD below.

Download PDF Download manufacturer protocol

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