APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- During the assay, cells were treated at different time intervals: 24, 48, and 72 hours.	- Fixed with 1% (w/v) paraformaldehyde in PBS for 15 min	- If signal intensity is low, increase incubation time for the DNA-labeling reaction

Upstream tips
- During the assay, cells were treated at different time intervals: 24, 48, and 72 hours.
Protocol tips
- Fixed with 1% (w/v) paraformaldehyde in PBS for 15 min
Downstream tips
- If signal intensity is low, increase incubation time for the DNA-labeling reaction

Publication protocol

Apoptotic cells were detected using the APO-BrDU TUNEL Assay Kit (Invitrogen). A549 cells were treated at different time intervals: 24, 48, and 72 hours. Cells were harvested, washed, and fixed with 1% (w/v) paraformaldehyde. The cells were then centrifuged, washed, and fixed with ice-cold 70% ethanol. DNA labeling was performed according to the manufacturer's instructions, and the cells were analyzed using Accuri C6 flow cytometer.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.