DeadEnd™ Fluorometric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent.

Downstream tips
- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent.

Publication protocol

The TUNEL assay was performed with cells cultured in chamber slides using the apoptosis detection kit (Promega) according to the manufacturer’s instructions. Slides were evaluated under a microscope (Olympus IX71) with a digital camera (Retiga 1300) interfaced to a computer with PCI software. The apoptotic cells were quantified as follows. The number of cells of the area (DAPI staining) were captured and counted. There were approximately 100 cells per area and three areas were captured in each slide. The fluorescence-labeled cells (apoptotic cells) from the same area were captured and counted. Three independent experiments were performed and analyzed.

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using DeadEnd™ Fluorometric TUNEL System from Promega.

Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download manufacturer protocol

Videos

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