ApoAlert™ DNA Fragmentation Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1.5 × 106 cells were platted for culture.		- No positive signal could be due to poor permeabilization with Triton X-100

Upstream tips
- 1.5 × 106 cells were platted for culture.
Downstream tips
- No positive signal could be due to poor permeabilization with Triton X-100

Publication protocol

The cells (1.5 × 106) were treated with different concentrations of Sub-A for 48 h. DNA was extracted and separated by 2% agarose gel electrophoresis. The gel was stained with 0.5 mg/mL ethidium bromide (EtBr), placed on a UV transilluminator, and then photographed.

Quantitative analysis was conducted by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) method using the BD ApoAlert DNA Fragmentation Assay Kit. The stained cells were analyzed by flow cytometer (Becton Dickinson and Co.) and fluorescence microscope (Nikon Eclipse TE 300, Germany) equipped with a filter system (emission filter 520 nm) at 20x magnification.

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Papers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ DNA Fragmentation Assay Kit below.

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Videos

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