MEBSTAIN Apoptosis TUNEL Kit Direct

Protocol tips

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	Add proteinase K and incubate for 30 min at 37C. Add TdT buffer and incubate for 5-10 min at RT.

Protocol tips
Add proteinase K and incubate for 30 min at 37C. Add TdT buffer and incubate for 5-10 min at RT.

Publication protocol

A MEBSTAIN Apoptosis Kit Direct (MBL International, Woburn, MA, United States) was used to perform TUNNEL staining according to the manufacturer’s protocol. Nucleosome-sized DNA fragments are detected by tailing their 3′-OH ends with digoxigenin nucleotides using terminal deoxynucleotidyl transferase (TdT). After treatment, the cells were combined with POD-horseradish peroxidase and incubated with the TdT buffer. The numbers of TUNNEL-positive cells was counted under a light microscope.

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Manufacturer protocol

Download the product protocol from MBL international corporation for MEBSTAIN Apoptosis TUNEL Kit Direct below.

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