MEBSTAIN Apoptosis TUNEL Kit Direct

TUNEL assay cell type - PANC-1 human pancriatic cancer

Experiment
TUNEL assay cell type - PANC-1 human pancriatic cancer
Product
MEBSTAIN Apoptosis TUNEL Kit Direct from MBL international corporation
Manufacturer
MBL international corporation

Protocol tips

Protocol tips
Add proteinase K and incubate for 30 min at 37C.

Add TdT buffer and incubate for 5-10 min at RT.

Publication protocol

A MEBSTAIN Apoptosis Kit Direct (MBL International, Woburn, MA, United States) was used to perform TUNNEL staining according to the manufacturer’s protocol. Nucleosome-sized DNA fragments are detected by tailing their 3′-OH ends with digoxigenin nucleotides using terminal deoxynucleotidyl transferase (TdT). After treatment, the cells were combined with POD-horseradish peroxidase and incubated with the TdT buffer. The numbers of TUNNEL-positive cells was counted under a light microscope.

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Discussion

Discussion

2 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - PANC-1 human pancriatic cancer using MEBSTAIN Apoptosis TUNEL Kit Direct from MBL international corporation.

Paper title
Apoptosis of human pancreatic carcinoma cell-1 cells induced by Yin Chen Hao Decoction
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Manufacturer protocol

Download the product protocol from MBL international corporation for MEBSTAIN Apoptosis TUNEL Kit Direct below.

Download PDF Download manufacturer protocol

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