p83Xi

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	The following steps are necessary for the functional overexpression of your Protein of Interest (POI) in S. cerevisiae (Figure 1): 1) cloning of the corresponding gene of interest (GOI) into a chimeric shuttle vector, 2) generation of S. cerevisiae competent cells, 3) transformation of GOI plasmid into S. cerevisiae competent cells, 4) cell growth (shake flasks or batch fermentation), 5) membrane preparations of S.cerevisiae, 6) expression confirmation of your POI via western blotting or GFP detection, and 7) expression optimization.

Protocol tips

The following steps are necessary for the functional overexpression of your Protein of Interest (POI) in S. cerevisiae (Figure 1): 1) cloning of the corresponding gene of interest (GOI) into a chimeric shuttle vector, 2) generation of S. cerevisiae competent cells, 3) transformation of GOI plasmid into S. cerevisiae competent cells, 4) cell growth (shake flasks or batch fermentation), 5) membrane preparations of S.cerevisiae, 6) expression confirmation of your POI via western blotting or GFP detection, and 7) expression optimization.

Publication protocol

The following steps are necessary for the functional overexpression of your Protein of Interest (POI) in S. cerevisiae (Figure 1): 1) cloning of the corresponding gene of interest (GOI) into a chimeric shuttle vector, 2) generation of S. cerevisiae competent cells, 3) transformation of GOI plasmid into S. cerevisiae competent cells, 4) cell growth (shake flasks or batch fermentation), 5) membrane preparations of S.cerevisiae, 6) expression confirmation of your POI via western blotting or GFP detection, and 7) expression optimization.

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