pSynInsPOT

Protein Expression Eukaryotic cells - S. cerevisiae Insulin

Experiment
Protein Expression Eukaryotic cells - S. cerevisiae Insulin
Product
pSynInsPOT from Jens Nielsen, Department of Chemical and Biological Engineering
Manufacturer
Jens Nielsen, Department of Chemical and Biological Engineering

Protocol tips

Protocol tips
CEN.PK530-1C was transformed separately with the POTud or CPOTud derived plasmids, and resulted in different engineered strains (Fig. 1 and Table I)

Publication protocol

CEN.PK530-1C was transformed separately with the POTud or CPOTud derived plasmids, and resulted in different engineered strains (Fig. 1 and Table I): strain AIP (with pAlphaInsPOT), SIP (with pSynInsPOT), AAP (with pAlphaAmyPOT), SAP (with pSynAmyPOT), AIC (with pAlphaInsCPOT), SIC (with pSynInsCPOT), AAC (with pAlphaAmyCPOT) and SAC (with pSynAmyCPOT). Blank plasmid CPOTud was also transformed to CEN.PK530-1C as the negative control (strain NC). For strains nomenclature see table I.

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