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Cells were grown in shaker cultures using Insect Xpress media (Lonza, Basel, Switzerland). To find the optimal expression conditions, cells at a density of either 1 or 2 million per milliliter were infected with baculovirus using a multiplicity of infection (MOI) of either 2 or 4, and cells harvested after 24, 48, or 72 h. |
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| Protocol tips |
| Cells were grown in shaker cultures using Insect Xpress media (Lonza, Basel, Switzerland). To find the optimal expression conditions, cells at a density of either 1 or 2 million per milliliter were infected with baculovirus using a multiplicity of infection (MOI) of either 2 or 4, and cells harvested after 24, 48, or 72 h. |
Publication protocol
Expression of the recombinant human MRP4-his6 within Sf9 cells was conducted using a baculovirus encoding for recombinant MRP4 generated from a pFastBac-MRP4-his6 construct as described previously.21 Cells were grown in shaker cultures using Insect Xpress media (Lonza, Basel, Switzerland). To find the optimal expression conditions, cells at a density of either 1 or 2 million per milliliter were infected with baculovirus using a multiplicity of infection (MOI) of either 2 or 4, and cells harvested after 24, 48, or 72 h.
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