pYT379-CDK8-CycC-10xHis complex

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	200 ml culture of Hi5 cells (1.0 × 106 cells/ml) or Sf9 cells (1.5 × 106 cells/ml) was infected with the amount of viruses indicated. Infected cells were incubated at 27°C for 96 hours, and were harvested by centrifugation, frozen in liquid nitrogen, and stored as a pellet at -80°C until future use.

Protocol tips
200 ml culture of Hi5 cells (1.0 × 106 cells/ml) or Sf9 cells (1.5 × 106 cells/ml) was infected with the amount of viruses indicated. Infected cells were incubated at 27°C for 96 hours, and were harvested by centrifugation, frozen in liquid nitrogen, and stored as a pellet at -80°C until future use.

Publication protocol

200 ml culture of Hi5 cells (1.0 × 106 cells/ml) or Sf9 cells (1.5 × 106 cells/ml) was infected with the amount of viruses indicated. Infected cells were incubated at 27°C for 96 hours, and were harvested by centrifugation, frozen in liquid nitrogen, and stored as a pellet at -80°C until future use.

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Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Eukaryotic cells - S. frugiperda CDK8-CycC-10xHis complex using pYT379-CDK8-CycC-10xHis complex from Yuichiro Takagi, Department of Biochemistry and Molecular Biolog.

Manufacturer protocol

Download the product protocol from Yuichiro Takagi, Department of Biochemistry and Molecular Biolog for pYT379-CDK8-CycC-10xHis complex below.

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Check out videos that might be relevant for performing Protein Expression Eukaryotic cells - S. frugiperda CDK8-CycC-10xHis complex using pYT379-CDK8-CycC-10xHis complex from Yuichiro Takagi, Department of Biochemistry and Molecular Biolog. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.