ApoBrdU Red DNA Fragmentation Kit

TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Experiment
TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer
Product
ApoBrdU Red DNA Fragmentation Kit from Biovision
Manufacturer
Biovision

Protocol tips

Upstream tips
- The experiments have been performed three independent times and in triplicates.
Protocol tips
-Cells were fixed in 1% (w/v) paraformaldehyde in PBS
Downstream tips
- Cells can be stored at –20°C for several days before use

Publication protocol

TUNEL assay was carried out to assess the DNA damage in HSP70-2 shRNA3 and shRNA4 treated cells using Apo-BrdU-Red in-situ DNA fragmentation assay kit (Biovision, Milpitas, CA, USA) as described earlier [14]. The experiments have been performed three independent times and in triplicates.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using ApoBrdU Red DNA Fragmentation Kit from Biovision.

Paper title
Heat shock protein 70-2 (HSP70-2) a novel cancer testis antigen that promotes growth of ovarian cancer
Full paper
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Manufacturer protocol

Download the product protocol from Biovision for ApoBrdU Red DNA Fragmentation Kit below.

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