pFlpBtM-II-eGFP

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	Sf21 cells were transfected with the respective expression plasmids at a density of 0.4–0.6x106 cells/mL using Lipofectin Transfection Reagent (Life Technologies). A DNA concentration of 2 μg per 1x106 cells was used at a Lipofectin: DNA ratio of 2:1. DNA and Lipofectin were incubated with the respective culture medium for precomplexing in 2.5% (v/v) of the final volume. After 30–60 min of incubation at RT the transfection mixture was diluted with medium in 37.5% (v/v) of the final volume and added to the cells.

Protocol tips

Sf21 cells were transfected with the respective expression plasmids at a density of 0.4–0.6x106 cells/mL using Lipofectin Transfection Reagent (Life Technologies). A DNA concentration of 2 μg per 1x106 cells was used at a Lipofectin: DNA ratio of 2:1. DNA and Lipofectin were incubated with the respective culture medium for precomplexing in 2.5% (v/v) of the final volume. After 30–60 min of incubation at RT the transfection mixture was diluted with medium in 37.5% (v/v) of the final volume and added to the cells.

Publication protocol

Sf21 cells were transfected with the respective expression plasmids at a density of 0.4–0.6x106 cells/mL using Lipofectin Transfection Reagent (Life Technologies). A DNA concentration of 2 μg per 1x106 cells was used at a Lipofectin: DNA ratio of 2:1. DNA and Lipofectin were incubated with the respective culture medium for precomplexing in 2.5% (v/v) of the final volume. After 30–60 min of incubation at RT the transfection mixture was diluted with medium in 37.5% (v/v) of the final volume and added to the cells.

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