In Situ Cell Death Detection Kit, Fluorescein

TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Experiment
TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer
Product
In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Downstream tips
- When there is a Nonspecific labelling, reduce concentration of TdT.

- When high background is present reduce concentration of labelling mix by 50%.

When low labelling is noticed, reduce fixation time.

Publication protocol

Cell death was assessed by trypan blue exclusion assay. Both floating and adherent cells were collected and stained with trypan blue dye for 5 min at room temperature. Cells were counted by four independent hemocytometer counts. Cell death was determined as the percentage of dead cells over the total number of cells. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was performed using In Situ Cell Death detection kit from Roche Applied Science following the manufacturer’s instructions

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich.

Paper title
Novel monofunctional platinum (II) complex Mono-Pt induces apoptosis-independent autophagic cell death in human ovarian carcinoma cells, distinct from cisplatin
Full paper
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

Download PDF Download manufacturer protocol

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