In Situ Cell Death Detection Kit, Fluorescein

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- When there is a Nonspecific labelling, reduce concentration of TdT. - When high background is present reduce concentration of labelling mix by 50%. When low labelling is noticed, reduce fixation time.

Downstream tips
- When there is a Nonspecific labelling, reduce concentration of TdT. - When high background is present reduce concentration of labelling mix by 50%. When low labelling is noticed, reduce fixation time.

Publication protocol

Cell death was assessed by trypan blue exclusion assay. Both floating and adherent cells were collected and stained with trypan blue dye for 5 min at room temperature. Cells were counted by four independent hemocytometer counts. Cell death was determined as the percentage of dead cells over the total number of cells. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was performed using In Situ Cell Death detection kit from Roche Applied Science following the manufacturer’s instructions

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.