CA125 Monoclonal Antibody (Ov185:1)

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Publication protocol

"Formalin-fixed paraffin-embedded tissue blocks of the LMS hysterectomy specimens of these patients were examined. Hematoxylin-eosin staining of 6 μm sections from the tumor tissue blocks were newly performed and reviewed by a senior pathologist (L.S.) in order to verify the diagnosis.
Immunohistochemistry was performed on additional 4 μm sections from each case. Anti-CA125, clone ZY-OV5 (Zymed Laboratories Inc. San Francisco, CA, USA) diluted 1:100 was used as a primary antibody. The staining included a retrieval process with CC1 solution for 64 minutes and detection by Ultra View Universal DAB Detection Kit (Ventana, Tucson, AZ, USA). The staining was performed on the Ventana Benchmark-Ultra automatic system. Sections of ovarian carcinoma known to express CA125 were used as positive controls.
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