LC3A (D50G8) XP® Rabbit mAb

Autophagy assay cell type - Vestibular schwannoma cells

Experiment
Autophagy assay cell type - Vestibular schwannoma cells
Product
LC3A (D50G8) XP® Rabbit mAb from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Upstream tips
Sub-confluent cells were treated with 0, 2.5 and 5μM of OSU-T315 for 48 and 72 hours for cell lines, and 72 hours for primary cells. Cells were then harvested and homogenized in lysis buffer (1% SDS, 10mM EDTA, 50mM Tris, pH 8.1) supplemented with protease/phosphatase inhibitor cocktail (Thermo Scientific, Waltham MA).
Protocol tips
Electrophoresis was performed with 25μg or 50μg of protein/lane in a 4-20% gradient SDS- polyacrylamide gel (Thermo Scientific, Waltham MA). Proteins were transferred to PVDF membranes (Millipore, Billerica MA), and probed with specific antibodies of interest and horseradish peroxidase-conjugated secondary antibodies.
Downstream tips
The chemiluminescent signals were detected in X-ray films.

Publication protocol

Sub-confluent cells were treated with 0, 2.5 and 5μM of OSU-T315 for 48 and 72 hours for cell lines, and 72 hours for primary cells. Cells were then harvested and homogenized in lysis buffer (1% SDS, 10mM EDTA, 50mM Tris, pH 8.1) supplemented with protease/phosphatase inhibitor cocktail (Thermo Scientific, Waltham MA). Electrophoresis was performed with 25μg or 50μg of protein/lane in a 4-20% gradient SDS- polyacrylamide gel (Thermo Scientific, Waltham MA). Proteins were transferred to PVDF membranes (Millipore, Billerica MA), and probed with specific antibodies of interest and horseradish peroxidase-conjugated secondary antibodies. The chemiluminescent signals were detected in X-ray films.

Full paper   Login or join for free to view the full paper.

Reviews

LC3A (D50G8) XP® Rabbit mAb from Cell Signaling Technology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Vestibular schwannoma cells using LC3A (D50G8) XP® Rabbit mAb from Cell Signaling Technology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3A (D50G8) XP® Rabbit mAb below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - Vestibular schwannoma cells using LC3A (D50G8) XP® Rabbit mAb from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms